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Utilization of chronopotentiometric titration in humic research
Viktorinová, Jana ; Pospíšilová, Ľubica (referee) ; Klučáková, Martina (advisor)
Humic acids are natural substances belonging to the group of humic substances. They arise mainly decomposition of plant residues. They are contained in soils, peat, sediments, young coal, water and even in the air. Humic acids are only partially soluble in water with increasing pH increases their solubility. Diploma thesis focuses on the use of chronopotentiometric titration of humic research. This method is mainly used for the determination of trace concentrations of analytes. This work is focused on the determination of acidity by potentiometric titration and the determination of dissociation constants using chronopotentiometry with measurement of pH of prepared samples.
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Determination of content of humic acids in solutions chronopotentiometrically
Viktorinová, Jana ; Kislinger, Jiří (referee) ; Klučáková, Martina (advisor)
Humic acids are part of humic substances, which are substances of natural origin occurring in soil, peats, sediments and young coal. These substances are mixture of compounds with different molecular weight and structures. Humic acids are only partially soluble in aqueous solutions and their solubility is growing up with icreasing pH value. Bachelor thesis uses method of chronopotentiometric titration, which has not been used yet for humic research. This bachelor thesis is trying to check possibility of exercise this method for assessment concentration of soluble humic acid. Especially trace amounts of analyt are measured by this method. Results obtained by chronopotentiometric titration are compared with results in common used method as UV/VIS spetctromerty and potentiometry.
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Catalytic and adsorption properties of papain and its derivatives
Lachmanová, Štěpánka ; Hudeček, Jiří (advisor) ; Karpenko, Vladimír (referee)
The aminoacid sequence of papain (EC 3.4.22.2) consists of 212 aminoacids. It has only one free sulfhydryl group, which is located in the active site of the protein. Some organometallic complexes could be bonded only to this free -SH group due to their structure. The artificial metalloproteins synthesised by this way may have different electrochemical properties. In this work, we have studied the electrochemical properties of papain and its derivatives. We compared the ability of papain and its three artificial derivatives to catalyse the hydrogen evolution by the chronopotenciometry. The work was completed by the study of the electrochemical properties of the organometallic complexes of ruthenium, which were used for the artificial metalloprotein preparation. The electrochemical properties of the compounds were never studied before. The process of the hydrogen evolution catalysed by the proteins is held in the adsorbed state of the catalyst. Due to this fact we have also studied the adsorption properties of papain on the substrates with different level of hydrofobicity. (In Czech)
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Catalytic and adsorption properties of papain and its derivatives
Lachmanová, Štěpánka ; Hudeček, Jiří (advisor) ; Karpenko, Vladimír (referee)
The aminoacid sequence of papain (EC 3.4.22.2) consists of 212 aminoacids. It has only one free sulfhydryl group, which is located in the active site of the protein. Some organometallic complexes could be bonded only to this free -SH group due to their structure. The artificial metalloproteins synthesised by this way may have different electrochemical properties. In this work, we have studied the electrochemical properties of papain and its derivatives. We compared the ability of papain and its three artificial derivatives to catalyse the hydrogen evolution by the chronopotenciometry. The work was completed by the study of the electrochemical properties of the organometallic complexes of ruthenium, which were used for the artificial metalloprotein preparation. The electrochemical properties of the compounds were never studied before. The process of the hydrogen evolution catalysed by the proteins is held in the adsorbed state of the catalyst. Due to this fact we have also studied the adsorption properties of papain on the substrates with different level of hydrofobicity. (In Czech)
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Utilization of chronopotentiometric titration in humic research
Viktorinová, Jana ; Pospíšilová, Ľubica (referee) ; Klučáková, Martina (advisor)
Humic acids are natural substances belonging to the group of humic substances. They arise mainly decomposition of plant residues. They are contained in soils, peat, sediments, young coal, water and even in the air. Humic acids are only partially soluble in water with increasing pH increases their solubility. Diploma thesis focuses on the use of chronopotentiometric titration of humic research. This method is mainly used for the determination of trace concentrations of analytes. This work is focused on the determination of acidity by potentiometric titration and the determination of dissociation constants using chronopotentiometry with measurement of pH of prepared samples.
|
|
|
Determination of content of humic acids in solutions chronopotentiometrically
Viktorinová, Jana ; Kislinger, Jiří (referee) ; Klučáková, Martina (advisor)
Humic acids are part of humic substances, which are substances of natural origin occurring in soil, peats, sediments and young coal. These substances are mixture of compounds with different molecular weight and structures. Humic acids are only partially soluble in aqueous solutions and their solubility is growing up with icreasing pH value. Bachelor thesis uses method of chronopotentiometric titration, which has not been used yet for humic research. This bachelor thesis is trying to check possibility of exercise this method for assessment concentration of soluble humic acid. Especially trace amounts of analyt are measured by this method. Results obtained by chronopotentiometric titration are compared with results in common used method as UV/VIS spetctromerty and potentiometry.
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